Monoclonal Antibodies Directed Against Subunits Of Human Rna Polymerase For Use In Immunofluorescence And Immunoprecipitation - Isis Project No 1138
Research at The Sir William Dunn School of Pathology, Oxford University, has resulted in the isolation of eight monoclonal antibodies directed against subunits of RNA polymerases I, II and III.
Background
During the final stages of the Human Genome Project, interest has increased in how information is transferred from genes to proteins, in which the RNA polymerases play a vital role. The nuclei of human cells contain three different types of RNA polymerase, characterised by their elution profile, drug sensitivity and location within the cell: polymerase I, II and III, each consisting of 12 to 17 subunits.
The Problem
Although the composition of RNA polymerases is the subject of constant study, the detection and localisation of many of the subunits has been restricted by the limited number of antibodies suitable for use in immunofluorescence and immunoprecipitation. Clearly it would be an advantage to know the function and location of all the subunits of such important cellular enzymes.
The Oxford Invention
Research at Oxford has generated eight monoclonal antibodies (mAbs) specific for different subunits of RNA polymerases which are suitable for use in immunofluorescence, immunoblotting and immunoprecipitation. Three mAbs are specific for subunits found in RNA polymerases, I, II, and III. Five further antibodies are specific for subunits of RNA polymerase III, and different ones are suitable for different applications. All have performed well in trials and, as RNA polymerases are highly conserved, these antibodies may cross react with the homologous subunits found in other eukaryotic cells.
Commercialisation Opportunity
Isis Innovation would welcome any inquiries from potential partners interested in utilising this technology.
Keywords
Antibodies, RNA Polymerase