CDK2/Cyclin A - Isis Project No 1441

Research within the Department of Biochemistry has lead to the production of an active CDK2/Cyclin A complex phosphorylated on Thr160 in a form suitable for crystallisation to allow studies into the interaction of the complex with potential ligands.

Background

CDK2 is one of the best-studied members of the cyclin-dependent kinase family, which plays a major role in eukaryotic cell cycle regulation. The activity of CDK2 is tightly controlled, requiring two inputs to become fully activated, namely the binding of cyclin A and the phosphorylation of threonine 160. Amongst other roles, fully activated CDK2 is essential for proper S phase progression. Studies have shown that inhibition of CDK2/cyclin A during S phase leads to S phase arrest and apoptosis, which has suggested a pharmacological role for CDK2/cyclin A inhibitors in the treatment of cancer.

Commercial Opportunity (Material Transfer)

Isis can provide phosphorylated CDK2/cyclin A in mg quantities. The potential applications of this compound include:

• Screening of compound libraries for potential ligands
• Crystallisation studies to assess the binding of compounds to the protein

Isis welcomes contact from companies interested in purchasing this protein.

Keywords

Protein, Oncology, Mitosis, Screen, Crystallization, Apoptotic

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